［Objective］Constructing a rational ecological network plays a crucial role in maintaining the sustainable development of regional ecological environment.［Methods］From the perspective of main functional zones，main functional scenarios with regional differences were established for three main functional zones. The PLUS model was used to simulate the land use patterns under the main functional scenario and the natural protection scenario in 2035. By coupling the InVEST and MSPA models，the ecological sources were comprehensively identified，and the ecological corridors were extracted using the circuit theory. The methods were used to construct multi-scenario ecological networks for Yunnan Province in 2020 and 2035，and evaluate the network structure. ［Results］1）Under the main functional scenario，cultivated land and forest land were effectively managed，and the regional expansion of construction land was strengthened，better meeting the needs of coordinated regional development. 2）The spatial distribution of ecological sources was similar under different scenarios，exhibiting a characteristic of“more in the west and less in the east”，with important ecological sources accounting for over 90% under each scenario. 3）In 2020，125 ecological corridors were constructed. In 2035，under the natural development scenario and the main functional scenario，the number of ecological corridors would be 113 and 133， respectively. The density of corridors showed a decreasing trend from southwest to northeast. 4）The ecological network structure indices under the main functional scenario were superior，indicating that the ecological network constructed based on this scenario is more ideal.［Conclusion］The proposed method and application of ecological network construction based on the main functional strategy can provide theoretical support and practical references for optimizing land space and building a "pioneer of ecological civilization " in Yunnan Province.