In order to explore the regulatory effects of indole-3-acetic acid (IAA) and kinetin (KT) on the physiological response and cadmium (Cd) tolerance of Helianthus tuberosus L. under Cd stress, taking H. tuberosus of Yulin and Chengdu as research objects, five treatment groups were set up, which were CK (no Cd), T1(Cd_{300 mg/kg}), T2(Cd_{300 mg/kg}+IAA_{4 mg/L}), T3(Cd_{300 mg/kg}+KT_{8 mg/L}) and T4(Cd_{300 mg/kg}+IAA_{4 mg/L}+ KT_{8 mg/L}), respectively, to study the effects of IAA and KT on the growth, antioxidant system, ChlorophyⅡ fluorescence properties and Cd accumulation ability of H. tuberosus seedlings under Cd stress. The results showed that the plant height elongation, leaf area, root elongation and dry weight of plants in the T1 group all decreased compared with CK. Spraying hormone could effectively increase chlorophyⅡ content, and the maximum increase was 162.87% in the T4 group of Yulin H. tuberosus. The proline (Pro) content of each group increased with the treatment time, the maximum increase of H. tuberosu T4 group in two places was 223.94% and 112.73%, respectively, while the decrease of MDA in T4 group was greater than that in T2 and T3.The antioxidant enzyme activity of T1 group was generally lower than that of other groups, and the gap in enzyme activities between groups was gradually widening with the plant growth. The increase ofthe three protective enzyme activities in T4 group was ideal. Hormone spraying significantly improved the root vigor of H. tuberosus (p<0.05), and the H. tuberosus T4 group in two places increased by 39.00% and 9.10%, respectively. In the treatment group of T4, the number of bubbles in the pith of H. tuberosus stem was much larger than that of T1 to T3, and the black area of plants from the two producing areas increased by 142.59% and 700.00%, respectively. Single or combined spraying of plant hormones could promote absorption of Cd in the root, stem and leaf organs of H. tuberosus, the content of Cd in root was the highest, followed by the stem. The Cd enrichment coefficient of each organ was all greater than 2, and transport coefficient was greater than 1. The Cd content of H. tuberosus T4 group was greater than T1 to T3.In summary, 4 mg/L IAA and 8 mg/L KT could help H. tuberosuseffectively respond to the Cd stress environment and strengthen its accumulation capacity by increasing the antioxidant enzyme activity and Pro content, so as to maintain the dynamic balance of reactive oxygen species (ROS) production and removal, reduce membrane lipid peroxidation, and improve root vitality. The results could provide theoretical guidance for the cultivation of H. tuberosus in Cd-polluted areas and phytore mediation of soil Cd pollution.