In order to study the soil anti-scourability and its relationship with soil physical properties in different land use types in karst area, taking Pinus Massoniana plantation land (PMP), Osmanthus fragrans plantation land (OFP), mixed forest land of natural arbor and shrub (ASL), natural grassland (NGL), abandoned land (AL) and cropland (CL) as research objects, the erosion test method of undisturbed soil flume was adopted, combining soil physical properties (bulk density, porosity, water-stable aggregates, mechanical composition, etc.), soil anti-scourability and its relationship with soil physical properties under different land use were quantitative analyzed. In the experiment, 3 slopes of 5°, 15°, 25° and 3 scour flows of 3.2, 4.8, 6.4 L/min were set up. The results showed that:(1) During the whole process of erosion test, the sediment concentration of runoff was the highest in the first 2 minutes of erosion, and then decreased gradually, and tended to be stable after 3~11 minutes. (2)The soil anti-scourability coefficient of each land use was NGL > PMP > ASL > AL > OFP > CL. The soil anti-scourability coefficient decreased with the increase of slope and erosion discharge, and the effect of slope on soil anti-scourability was more obvious than that of erosion discharge; (3) Soil anti-scourability was significantly positively correlated with soil bulk density, sand content and water-stable aggregate content (P < 0.01), and negatively correlated with total porosity and clay content (P < 0.05), which could be expressed by power function (R²>0.78). The results can provide scientific basis for soil erosion research and soil and water conservation control in karst area.