In order to explore the effect of freeze-thaw cycle on the ability of black soil aggregate to resist the influence of different damage. the typical black soil of Heilongjiang province was taken as the research object. and the effects of freeze-thaw cycles and the initial moisture content on stability of soil aggregate with different initial size were analyzed through three different crushing processes of LB method. The results showed that the freeze-thaw cycles had significant effect on the stability of aggregates with different size of 1~2, 2~3 and 3~5 mm (P<0.05). The stability of aggregates followed the order of wetting oscillation (WS) > slow wetting (SW) > fast wetting (FW). FW treatment had the greatest damage to the stability of black soil aggregates, while the SW treatment and WS treatment had less damage. At the same time, the initial water content and the number of freeze-thaw cycle had extremely significant influence on the MWD_FW, MWD_SW and MWD_WS of three different sizes aggregates (P<0.01). With the increasing of the number of freeze-thaw cycle, the large aggregate of >0.2 mm was broken and the content of the small aggregate (<0.2 mm) increased. The initial water content had a destructive effect on 2~3 and 3~5 mm aggregates, while for 1~2 mm aggregates, it was helpful to improve the structure and increase the stability of aggregates.