In order to explore the changing law of soil properties in different degraded alpine meadows, we measured and analyzed the soil nutrient content and ecological stoichiometric characteristics of different degraded alpine meadows (mild degraded grassland, moderate degraded grassland and severe degraded grassland) located in Kangle, Huangcheng and Tianzhu experimental plots in the Qilian Mountains. The results showed that: (1) The soil pH, electrical conductivity, water content and organic matter content decreased with the increasing of soil depth. In the 0—20 cm soil layer, the soil pH showed an upward trend, and the electrical conductivity increased first and then decreased. In the 20—40 cm soil layer, the soil pH increased first and then decreased, while the electrical conductivity showed the opposite trend. Soil waters and organic matters in 0—20 and 20—40 cm soil layers decreased gradually as the degree of degradation intensifying. (2) The contents of organic carbon, total nitrogen, total phosphorus and total potassium in different grassland soil decreased gradually with the increasing of degradation degree and soil depth, and the differences among plots were significant (p< 0.05). (3) In different soil layers of different degraded grasslands, the variation range of C/N was 19.10 to 40.48, the change range of C/P was from 87.85 to 121.97 and the range of N/P changed from 4.10 to 6.76. (4) As the intensifying of grassland degradation degree, the soil cellulase activity increased first and then decreased, urease and neutral phosphorus acid enzyme, dehydrogenase, invertase and oxidoreductase showed a decreasing trend. (5) The results of correlation analysis between soil physicochemical, enzyme activity and ecological stoichiometric ratio and principal component analysis showed that the soil physicochemical index, enzyme activity and ecological stoichiometric ratio could reflect the soil quality of different degraded grasslands. In conclusion, the soil of degraded alpine grassland had deteriorated gradually in Qilian Mountains. The rational utilization and scientific management of grassland in this area should be strengthened.