The objectives of this study were to investigate the influences of different concentrations of zinc (Zn) application on cadmium (Cd) bioavailability in soil and Cd accumulation in rice grown in two Cd-polluted soils. Rice pot experiment was carried out and the rice variety was Weiyou 46. Application exogenous Cd and Zn,curing generated two Cd-polluted soil (0.72 and 5.26 mg/kg Cd) and four Zn-treated soil (0, 40, 80 and 160 mg/kg). Results showed that the application of Zn had an influence on the test indexes, while soil Cd concentration was the dominant factor affecting the soil Cd activity and the Cd accumulation in the rice plants. In the 0.72 mg/kg Cd treatment, the application of Zn reduced exchangeable Cd concentration in soil by 1.9%~17.0%, while increased the uptake and accumulation of Cd in the iron plaque, roots, and brown rice, the Cd concentration in brown rice was ranging from 0.09 to 0.17 mg/kg, furthermore, the Cd concentration in brown rice was linearly positively related to the exchangeable Zn concentration in soil. In the 5.26 mg/kg Cd treatment, the exchangeable Cd concentration in soil was increased by 2.1%~4.8%, the Cd concentration in various rice organs was decreased. Moreover, the Cd concentration in brown rice decreased to 0.12 mg/kg when the Zn concentration increased to 80 mg/kg, whereas it was 0.45 mg/kg at 0 mg/kg Zn, lower than the National Food Safety Standard of 0.2 mg/kg (GB 2762-2017). In addition, the correlations of the Cd concentration in brown rice and exchangeable Zn in soil were linear and negative. The experiment results also found that application of Zn increase the accumulation rate of Cd in the underground part of rice planed in two Cd-polluted soils, and then decreased the Cd accumulation rate of aerial part. A certain amount of Zn to reduce the Cd concentration in brown rice was feasible in the soil treated with 5.26 mg/kg Cd and the amount of Zn 80 mg/kg was the best applied in the experiment, while it increased the risk of Cd accumulation in the soil treated with 0.72 mg/kg.