This paper studied the dynamics of soil aggregation in responses to organic materials and their incorporation methods through a laboratory cultivating experiments. Five treatments were involved: no addition as a control (CK), mixing straw (M-Str), straw mulching (Str), biochar (BC) and swine manure (SM). The results show that the M-Str and SM treatments significantly increased soil organic carbon (SOC), hot water extractable carbon (HWC), tensile strength (TS) and mean weight diameter (MWD) of aggregate stability (p＜0.05), whereas the Str treatment did not enhance SOC and MWD (p＞0.05). Compared to the CK treatment, the BC treatment did not improve MWD although it increased SOC significantly (p＜0.05). The aggregate stability in the M-Str and SM treatments was linearly related to soil respiration (CO2) (p＞0.05). Our results demonstrate that organic materials and their incorporation methods played a critical role in soil aggregation stability.