In order to reveal the influences on the runoff producing and sediment on the hillslope which were exerted by different tillage measures in Karst region, the researchers used independent designed soil storehouse and indoor simulated rainfall measures, set particular rainfall intensity to study the features of runoff and the soil loss of the naked slope farmland in Karst region. The results showed: When the rainfall intensity was 63 mm/h and the declivity was less than 10°, ploughing measures could decrease the time of runoff producing of underground pores, and no-till could slow down its runoff producing, and its sediment yield was 66% of no-till. Otherwise, it conversed when the declivity was greater than 10°, and the sediment yield of ploughing were 16% and 6% of no-till separately. When the rainfall intensity was 100 mm/h and the declivity was less than 10°, the ploughing measures could speed up the confluence process of surface runoff on slopes, and the sediment yield of no-till was 41% of ploughing. Otherwise, it conversed when the declivity was greater than 10°, and the sediment yield of ploughing were 68% and 0.9% of no-till separately. In the circumstance of the same declivity and rainfall intensity, ploughing measures could decrease the loss of soil with surface runoff.