To explore the various degree water input influence on soil respiration in subtropical monsoon climate region, selected mixed broadleaf-conifer forest of Chongqing Jinyun Mountain Nature Reserves as the research object in 2014. The artificial blocking and increasing water were used to simulate different precipitation input, and then to analyze the impact (including 70% of saturated soil water content (addition 70%: 70%A), 100% (addition 100%: 100%A), 130% (addition 130%: 130%A), comparison (CK) and no precipitation (Z)). The result shows that: (1)All the diurnal changes of soil respiration formed a curve under the different water inputs, and it reached the maximum value during 12:00 to 14:00; and the same as monthly variation, it reached the maximum value in August and the minimum value in December; (2)Properly increased water could improve the Pearson correlation between soil respiration rate and soil temperature of 10 cm soil depth, but the best fitting of exponential model was comparison group. Reducing water and excessive increase water would reduce its correlation; the same as the temperature-sensitivity coefficient Q10; (3)The correlation of soil respiration rate and soil water content of 10 cm soil depth was low as a whole, but the influence was obvious. In addition to no precipitation showed significantly positive correlation, it almost off the mark between comparison and water content, and it negatively correlated between water addition and water content, especially the addition 130% was marked negative correlation; (4)In General, water addition 100% improved the soil respiration rate, expanded its excursion, water reduction reduced the soil respiration rate in the study region nature environment.