Influence of manure application and copper input on copper fractionation and its biological effects were studied in this paper.The results showed that manure application and copper input all could affect the distribution of copper among different fractions.With the increase of copper input,the exchangeable copper only had a slight increase,while the other fractions increased rapidly.The relative amount of organically bound and manganese oxide bounded copper increased more rapidly.In the 200 mg/kg copper tre...