Based on the long-term position experiment in Hailun Agricultural Ecology Station of Chinese Academy of Sciences,the characteristics of soil fertilities in black soil under different land uses and management were investigated.The results showed that there was great significance among the soil fertilities under different land uses.Soil fertility in NF sharply decreased cause of removal of vegetation.Soil total C,total N and available N in natural restoration were enhanced significantly.Soil fertilization management could prevent the decreasing of soil fertility.Organic manure increased the content of soil nutrition pools,and improving soil fertility.There may be qualities unique to each cover crop that increasing the activity of special enzymes.Special factors of different land uses could influence different soil enzymes activities.Natural restoration influenced the activity of invertase and phosphatase more than soil fertilization(C,N and P).Both natural vegetation restoration and long-term fertilization are importance in increasing soil organic C and N pools,and improving soil fertility.