Annualized Agricultural Non-Point Source model(AnnAGNPS),a continuous-simulation,watershed-scale model,is widely used to evaluate non-point source pollution from agricultural watersheds in a number of countries.AnnAGNPS simulates surface water,sediment,nutrients,and pesticides leaving the cells and their transport through the watershed.The Daning river watershed was selected as the case study.Based on 3S(GIS,Remote Sensing,Global Position System) technology,the AnnAGNPS model database was set up in the watershed by analysis of rainfall characteristics,size of simulating cells,translation of soil relative parameters,characteristic of land use management,and so on.The parameters on climate,topography,soil,landuse,crop information and crop management were evaluated.This study was expected to provide a sound and credible basis for decisions to evaluate soil erosion,sediment and agricultural non-point source pollution in Daning river watershed and Three Gorges Reservoir Region.