Field experiment and laboratory simulated experiment were conducted to study the dynamic process of soil respiration flux,SMBC,basal respiration,metabolic quotient(qCO2) and DOC for Doyeuxia augustifolia wetland and cultivating-ten-year soil(agricultural soil) after tillage.The results showed that soil water content of Doyeuxia augustifolia wetland decreased significantly after soil tillage.Soil CO2 flux reached a peak in primary 1~2 days after tillage.CO2 flux of cultivated soil with tillage was higher all the time than that of soil without tillage(p<0.01).SMBC content of agricultural soil was higher significantly than that of Doyeuxia augustifolia wetland(p<0.001).SMBC content of wetland and agricultural soil had no significant difference in the primary 1~3 days after tillage.Since then SMBC content of soil with tillage increased rapidly and was higher than that of soil without tillage.For agricultural soil,SMBC content of soil with tillage was higher all through than that of soil without tillage. Soil tillage had no significant influence of DOC content on agricultural soil. However,DOC content of wetland increased rapidly after tillage.For wetland,DOC content of tillage was higher significantly than that of no tillage in primary 80 days(p<0.01),while lower than that of no tillage after 80 days.