Experiments were carried out to study the effect of green manure application on soil chemical,physical and biological properties in tobacco field of Xiangcheng,Wuyang,Yexian and Luoshan in Henan province.The green manures sowed between the September and October in 2003 and the application of green manures was in the March of 2004.The treat ment of each green manure is 1/3 hm~2,and 1/15 hm~2 is the contradistinction in it.Results indicated that the application of green manuresmay decrease the soil bulk density affectively and adjust the soil pH,meanwhile it may also result the soil microbial biomass to increase greatly. The result shows Soil bulk density can be reduced 0.03~0.1 g/cm~3,the soil pH can be reduced 0.1~0.6 and soil organic matter can be increased 0.08%~0.1% with the application of green manures.Thus,application of greenmanures may enhance the soil fertility and be favorable to the sustainable development of tobacco production.