This paper analyzed the daily variability and the seasonal variability and spatial variability of the transpiration rate of four kinds of bushes in soil and water conservation, and the correlation analysis and multi-regression analysis were applied to their transpiration rate and influencing factors. The results of the study were the following. The total tendency of the daily variability of four kinds of bush transpiration rate was that it was relatively small in early morning and it reached peak value basically at about 12:00 at noon and dropped to the utmost point from side to side low by 18:00. The change had certain differences on different bushes. According to peak value of the transpiration rate, the size order of the transpiration rate of the four kinds of bushes in dry season was Vitex negundo Linn.> Cotinus coggygria Scop. > Forsythia suspense (Thunb.) Vahl.> Spriaea trilobata Linn.,and it was Vitex negundo Linn. > Spriaea trilobata Linn. > Forsythia suspense (Thunb.) Vahl.> Cotinus coggygria Scop. in rainy season. The variability tendency of season of four kinds of bush transpiration rate was the same basically. It showed a tendency to reduce in May and then went up gradually since the beginning of June and reached the maximum in mid-July and then began to fall after rise shortly afterwards. The characteristic of spatial variability of transpiration rate was that the largest transpiration rate on top and the middle part takes second place, and the under-part is minimum. The transpiration rate of four kinds of bushes extremely remarkable had with the effective radiation, temperature, air relative humidity and air vent is it spends four extremely remarkable correlations.