Through analyzing the changes of proline,soluble saccharin and endogenous hormone contents in leaves of Chinese seabuckthorn and Russian seabuckthorn under drought stress,the results showed that the slope soil's water content is low than the gully, the proline content and the soluble saccharin content in leaves of Chinese seabuckthorn in the slope increased than it in the gully. In the field drought stress,the content of proline and soluble saccharin increased with decreasing of water content of the soil.The ability of accumulation of the proline of Chinese seabuckthornis more than of Russian seabuckthorn,and the female more than the male. The ability of accumulation of the soluble saccharin of Russian seabuckthorn is more than of Chinese seabuckthorn,and the female more than the male. The content of ABA increased with decreasing of water content of the soil.The content of IAA and ZR decreased with decreasing of the water content of soil.The content of GA increased in the initial stage of drought stress,then decreased after the initial stage. From the changes of proline content,soluble saccharin content,endogenous hormone content can conclude that the Chinese seabuckthorn and the female of seabuckthorn have high adaptability to water stress more than the Russian seabuckthorn and the male of seabuckthorn.