Effects of different methods of application on physical, chemical (soil nutrient) and biological property of degenerated soil were studied in middle part of Tibet. Preliminary results showed that there was marked effect of apply organic manure, especially the organo mineral fertilizer could reduce the bulk density, and improve the porosity, 1~3mm waterstable aggregate, soil nutrient contents, organic matter contents, amount of earthworm under the ecological conditions of the Tibet plateau in a short time, and these increased significantly by the increase of the amount of organic manure. But comprehensive effect of chemical fertilizer (consist of balance manuring) was lower relatively. The organic matter and available potassium increased by a big margin will were vital question in the course of the fertility restoration of degenerated soil. The amount of bacterium account for overwhelming majority of the soil microbes under the different methods of application, and it made a greater contribution to crop nutrition. Soil fungus and actinomyces was relatively less under the applied inorganic fertilizer and organo mineral fertilizer.