[Objective]Driven by global climate change and the continuous intensification of human social and economic activities, we explored the imbalance between supply and demand of ecosystem services in Anhui Province, and provided reliable basis for regional ecological security construction.[Methods]Anhui Province was selected as the research area, and the InVEST model and hotspot analysis method were integrated to systematically evaluate the supply and demand capacity of the four key services of water yield, habitat quality, carbon storage and soil conservation and the comprehensive cold hotspot distribution pattern in Anhui province from 2003 to 2023. From the perspective of ecosystem service supply and demand, we constructed the ecological supply and demand pattern of Anhui province with the help of circuit theory[Reults]1) From 2003 to 2023, water yield would first decrease and then increase, with a spatial distribution of "more in the south and less in the north". Carbon storage showed a decreasing trend. The habitat quality was higher in the south than in the north and decreased year by year. The amount of soil retention decreased first and then increased, with an overall upward trend.2) From 2003 to 2023, the demand for water production service, carbon sequestration service and habitat demand would generally show an upward trend. The overall demand for soil conservation services (soil erosion) in Anhui province showed a significant downward trend.3) The hotspots of comprehensive ecosystem services supply in Anhui Province were characterized by "high in the south and southwest, and low in the north". The hot spots of comprehensive demand were high in the northwest and low in the southeast. The comprehensive supply-demand ratio showed the characteristics of "high in the south and low in the north".4) The research consensus included 40 supply sources and 36 demand sources; There were 93 supply corridors and 96 demand corridors. Forming an ecological supply and demand pattern of "southern screen and northern link, river basin through".[Conclusion]From the perspective of supply and demand relationship, we constructed a multi-objective coordinated ecological supply and demand network, provided scientific basis for the optimal allocation of regional resources and sustainable ecosystem management, and formed a decision-making support system with both theoretical value and practical guidance.