[Objective] To investigate the elevation-based differentiation in moisture and nutrients of sedimentary layer in the water-level fluctuation zone（WLFZ）and provide a scientific basis for reservoir management. [Methods] The WLFZ of the Three Gorges Reservoir was selected as the research object and layered sample were collected from a representative slope. The particle size distribution，nutrient concentration，and moisture content were measured，and the differences in sediment properties at different elevations were analyzed. [Results] 1）The particles of the sediment in the WLFZ were relatively fine，with a median particle size ranging from 6.1 to 13.5 μm and an average of 8.7 μm. No significant difference in median particle size was observed among sediments at varying elevations（p>0.05）. The particle size distribution of the sediments was dominated by silt，with an average volume content of 82.7%，and the contents of clay and sand were 14.2% and 3.1%，respectively. 2）The mean total organic carbon（TOC），total nitrogen（TN），and total phosphorus（TP）contents of the sediments in the WLFZ were 18.82，1.24 and 0.75 g/kg， respectively. The stoichiometric ratios of carbon，nitrogen，and phosphorus（C∶N，C∶P，and N∶P）were 15.25， 25.26 and 1.68，respectively. Significant differences were observed in the overall trends of nutrient content changes with elevation. Specifically，TOC and TN contents increased significantly with increasing elevation（p<0.05），while TP content did not show a clear trend. The stoichiometric ratios of C∶P and N∶P in the sediments increased with increasing elevation，while the C∶N ratio showed no significant trend（p>0.05）. 3）The average mass water content of the sediments in the WLFZ was 28.63%，and no significant elevation-based spatial differentiation was observed（p> 0.05）. 4）The particle size distribution，nutrient content，and moisture content of sediments in the WLFZ were distributed in striated layers formed by alternating high and low values along the profile. [Conclusion] There is a significant elevation-based differentiation in the nutrient content of the sediments in the WLFZ of the Three Gorges Reservoir，but there is no significant spatial differentiation in particle composition and mass water content.