Habitat quality is an important reference index to measure the quality of a region’s ecological environment, and research on land use change can provide a scientific basis for evaluating the spatio-temporal dynamic evolution characteristics of regional habitat quality. Based on the land use data in 1980, 2000 and 2018, this paper analyzes and predicts the change of land use in Yulin City through the InVEST model, PLUS model, and geographic information map method, so as to explore the spatio-temporal evolution characteristics of habitat quality in Yulin City, Shaanxi Province. The conclusions are as follows: (1) The landscape matrix in Yulin City is mainly composed of grassland and cultivated land, followed by forest land and unused land, while water and construction land account for a relatively small proportion. The implementation of the policy of returning farmland to forest, the development of desertification control projects, and the development of construction land are the main driving factors of land use change. (2) From the overall analysis of the region, the average habitat quality in 1980, 2000, 2018 and 2030 is 0.502 8, 0.520 9, 0.518 3 and 0.525 9, respectively. The overall habitat value does not change much, showing a fluctuating trend of first increase, then decrease and then increase. From the analysis of the spatial pattern, the distribution of habitat quality in Yulin City has a certain regularity, and the overall pattern is high in the east and low in the west. The average value of habitat degradation in Yulin City is 0.029 8, 0.028 9, 0.029 2 and 0.028 3, respectively, indicating the threat of habitat degradation. The research results have theoretical support and policy significance for the protection of the natural environment and the improvement of habitat quality in Yulin City.