Forest soil is the basis for the survival and restoration of forest vegetation. Disturbance not only affects the dynamic changes of forest soil organism sand abiotic, but also has an important impact on the structure and function of forest ecosystem. The study selected three types of disturbance (clearing litter, resin tapping and fire) Pinus massoniana forests in central Guizhou as the research object, and set up undisturbed P. massoniana forest plots around each of the disturbance plots as the control, to reveal the influence characteristics of different disturbance types on soil properties of P. massoniana forest. The results showed that there were significant differences in the soil properties of the P. massoniana forest among different soil layers (P<0.05), and the nutrient content of the top soil (0—20 cm) was significantly higher than that of the lower soil (20—40 cm and 40—60 cm), which indicated that the soil nutrients of the P. massoniana forest had "surface accumulation" effect. Different disturbance types had a significant impact on the soil properties of the P. massoniana forest (P<0.05). Under the disturbance of resin tapping, soil nutrient content and urease activity increased, and there was no significant difference in contents of total phosphorus and available phosphorus. Under the disturbance of litter removal, the soil pH, organic carbon content, total nitrogen content and urease activity of the P. massoniana forest decreased, and there was no significant difference in the bulk density, total phosphorus content, available nitrogen content and available phosphorus content, while the available potassium content and catalase activity increased. Under fire interference, the bulk density, available potassium content and catalase activity of the P. massoniana forest increased, while the contents of organic carbon, total nitrogen, total phosphorus and available nitrogen, as well as urease activity showed a downward trend, while the contents of organic carbon and available phosphorus did not unchanged significantly. Under different interference types, there was a significant correlation between soil enzyme activity and chemical properties. The significant correlation in surface soil was greater than that in deep soil. Urease activity was significantly correlated with contents of total nitrogen and available nitrogen, and catalase activity was significantly correlated with organic carbon content. Under different interference types, there were certain differences in soil nutrient variability. The soil pH was weak variability, and most of the remaining soil nutrients were medium variability. In summary, different types of disturbance had a great impact on the soil properties of the P. massoniana forest. Sufficient attention should be paid to the utilization and protection of the P. massoniana forest in this area, and rational management and utilization should be given.