Soil enzymes are closely linked with nutrients and chemical properties of soil. Soil enzymes can activate nutrient elements in soil to improve soil quality. Changes in net primary productivity in response to climate change are likely to affect litter inputs to forest soil. However, the effects of altered litter input on soil enzymes activities and their stoichiometry remain poorly understood in Castanopsis carlesii forest. In this study, soil hydrolases β—glucosidase enzyme (βG), cellulose (CBH), β—N—acetylglucosaminidase (NAG), leucine aminopeptidase (LAP), and acid phosphatase (AP) activities and their stoichiometric characteristics under different litter input treatments with root exclusion, viz. no litter (NRNL), double litter (NRDL), control (CK) were measured in a subtropical Castanopsis carlesii forest in Sanming, Fujian, China. The results showed that compared with CK, NRDL significantly improved CBH and LAP activities, and increased the contents of soil total carbon (TC), total nitrogen (TN), mineral nitrogen, dissolved organic carbon (DOC), and microbial biomass nitrogen (MBN), while the content of MBC was only reduced under NRNL. It was also found that the soil enzyme stoichiometric ratio was not affected by litter input treatments. Moreover,the values of ln (βG+CBH)∶ln (NAG+LAP) were 1.25, 1.36, 1.30; the values of ln (βG+CBH) ∶ln (AP) were 0.78, 0.78, 0.83; the values of ln (βG+CBH) ∶ln (NAG+LAP) were 1.25, 1.36, 1.30; ln (NAG+LAP)∶ln (AP) were 0.62, 0.58, 0.64. The correlation analysis showed that TC, TN, mineral nitrogen, DOC, DON, MBC and MBN were significantly positively correlated with βG,CBH,AP and LAP activities. Redundancy analysis indicated that the changes in hydrolases activities and their stoichiometry were mainly driven by soil DOC concentration. Thus, NRDL could improve the hydrolase activity by increasing the concentration of soil DOC. This study could provide a basic theory for soil nutrient management in subtropical forests.