Mountainous area accounts for about 94% total land area of Yunnan province. The special topographical characteristics can easily cause slope soil erosion and nutrient loss, which seriously affects agricultural sustainable production. It is necessary to take effective agronomic measures to reduce soil nutrient loss in sloping land. Based on the 4-year experiment, compound agronomic measures for two tillage directions, vertical ridge (VR) group (2 treatments) and horizontal ridge (HR) group (2 treatments) were in location monitoring. Results showed that runoff generation time of red soil on slope land in Yunnan was mainly from June to September, and the rainfall of producing runoff accounted for 65.62%~75.82% of the annual precipitation. The rainfall of producing runoff had same trend with annual precipitation. The relationship between rainfall of producing runoff and runoff depth were linear (R_NVF²=0.597 7,R_OVF²=0.415 1,R_OHF²=0.378 2, R_OHFR²=0.335 5).The correlation order was that vertical ridge (VR) group was greater than horizontal ridge (HR) group, non-fertilizer application treatment was greater than fertilizer application treatment, and mulching treatment was greater than the mulching removal treatment (P<0.01). Heavy rain and rainstorm caused most of the annual runoff and nutrient loss. Surface runoff produced by HR tillage was (177.13±28.87) to (182.28±33.75) mm, TN (7.66±2.51) to (7.85±1.92) kg/hm², TP (0.91±0.26) to (1.09±0.27) kg/hm². Average reduction rate (ARR, %) of runoff and nutrients were 49.57% to 52.13% and 33.16% to 53.88% significantly, compared with conventional treatment (OVF) of VR tillage group(P<0.01). Under different tillage measures, OHFR had best effect of intercepting runoff and nutrient losses. The results of RDA analysis show that compared with rainfall, runoff was the main environmental factor affecting the change of nitrogen and phosphorus nutrient (P<0.01). The correlation between runoff and the nitrogen nutrient loss (except NH₄⁺-N) was higher than that of phosphorus, which indicating nitrogen was more easily loss with runoff than phosphorus. NVF and OVF treatments of vertical ridge (VR) group were positively correlation with environmental factor runoff and nutrient losses of TN、TDN、NO₃^--N、NH₄⁺-N、TP、TDP, while OHF and OHFR treatments of horizontal ridge (HR) group were negatively correlation with it.