解磷、钾功能性微生物耐盐效应研究
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耿丽平(1984-),女,硕士研究生,讲师,主要从事土壤环境质量研究。E-mail:genglip486@126.com

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河北省应用基础研究计划重点基础研究项目(17962902D);国家科技支撑计划项目(2015BAD23B01);河北省农业产业体系项目(HBCT2018030206)


Study on Salt Tolerance of Functional Microbes with Ability to Dissolve Insoluble Phosphate and Potassium
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    摘要:

    围绕设施菜田土壤盐渍化问题,以解磷、钾功能微生物胶质芽孢杆菌和巨大芽孢杆菌为研究对象,探究了盐分胁迫对功能菌活性、解磷钾能力的影响,并阐明了含2种功能菌微生物菌剂在轻度盐渍化设施菜田的作用效应。结果表明,混合功能菌最大耐受盐浓度为10.5% NaCl,最适宜生长盐分浓度为0.5% NaCl,且解磷、钾能力最强,有效磷、钾增幅分别为52.06%,75.41%;此外,轻度盐渍化设施菜田的小区试验结果显示含2种功能菌的菌剂可提高番茄、甜瓜、西瓜果实VC和可溶性糖含量,分别增产104.81%,23.72%,28.96%;土壤中有效磷和速效钾含量分别提高95.12%,22.83%,134.52%和92.71%,6.66%,117.46%,显著增加了土壤菌群数量(P<0.05)。综上所述,解磷、钾功能微生物具有耐盐性,可在轻度盐渍化土壤中定殖,并能活化土壤磷、钾,提高蔬菜产量和改善品质。

    Abstract:

    Based on soil salinization in vegetable fields of greenhouse,two bacteria (Bacillus mucilaginosus and B. megaterium that can dissolve the insoluble phosphate and potassium) were taken as study objects,microbial incubation and field experiments were conducted to investigate the effect of salt stress on the activity and the phosphate and potassium solubilization of two bacteria,and verify the effects of microbial inoculum application in lightly salinized greenhouse soil. The results showed that the maximum tolerated salt concentration was 10.5% NaCl and the optimum salt concentration of mixed bacteria was 0.5% NaCl,the available P and K concentrations dissolved from minerals were highest in growth medium with 0.5% NaCl and increased by 52.06% and 75.41%. The application of microbial inoculum in the vegetable field with high soil salinization significantly increased the levels of VC and soluble sugar in fruits of tomato,sweet melon,and water melon,and obtained greater yields with increment of 104.81%,23.72%,and 28.96% compared to the control. Moreover,the application of microbial inoculum significantly increased the concentrations of soil available phosphorus and potassium from the fields with tomato,sweet melon,and watermelon by 95.12%,22.83%,134.52%,and 92.71%,6.66%,117.46%,respectively,and significantly increased the number of soil bacteria (P<0.05). In conclusion,microbial inoculum with B. mucilaginosus and B. megaterium can colonize in slightly salinized soil,promote the release of te phosphorus and the potassium in the soil,increase vegetables yields,and improve the quality of vegetable fruits.

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耿丽平, 范俊, 王婧瑶, 赵全利, 薛培英, 刘文菊.解磷、钾功能性微生物耐盐效应研究[J].水土保持学报,2020,34(4):370~375

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  • 收稿日期:2019-11-10
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  • 在线发布日期: 2020-08-04
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