In order to provide a scientific basis for the cultivation and breeding of wheat and the improvement of nitrogen utilization in central Shandong. "Tainong 18" "Linmai 4"and "Wennong 5"which are three high-quality wheat varieties with a wide cultivation area in central Shandong were selected and three nitrogen levels of 0,120,240 kg/hm² were set to study the response of yield and nitrogen utilization of different wheat varieties to nitrogen application rate with field experiment for 2 years. The results showed that the application of nitrogen fertilizer could increase the synthesis and accumulation of photosynthetic pigments in wheat leaves,promote photosynthesis,increase the dry matter accumulation of wheat and its transport to grain,facilitate the construction of wheat population and promote the formation of yield. The yields of three wheat varieties at the N120 and N240 level respectively increased by 0.67~2.10 times and 0.97~2.62 times compared with the N0 level. With the increase of nitrogen application rate,the responsiveness of wheat to nitrogen fertilizer decreased,nitrogen absorption and utilization efficiency,nitrogen fertilizer partial productivity and agronomic efficiency were significantly reduced. Variety differences have significant effects on wheat growth and yield formation. In this study,all indicators of wheat showed "Tainong 18" > "Linmai 4" > "Wennong 5". "Tainong 18" had the highest yield and better nitrogen absorption and utilization ability. "Wennong 5" was the most sensitive to nitrogen. Compared with "Linmai 4" and "Wennong 5",the yield of "Tainong 18" increased by 15.83%~26.42% and 34.50%~42.43% respectively under the N0 level,increased by 4.97%~5.05% and 12.23%~12.54% respectively under the N120 level,increased by 4.13%~5.75% and 8.49%~11.08% respectively under the N240 level. In summary,the optimum nitrogen application rate in central Shandong is 240 kg/hm² under the conditions of this experiment,"Tainong 18" is the most suitable wheat variety for promotion,and "Wennong 5" has great potential for increasing yield which can provide a scientific basis for wheat variety selection.