In order to screen out the passivator combination of organic and inorganic materials, which could effectively inhibit the absorption and accumulation of chromium (Cr) and copper (Cu) in various parts of rice, a pot experiment in paddy soil was conducted to study the effects of 15 combinations of passivators on occurrence forms of Cr and Cu in soil and absorption and accumulation of Cr and Cu in rice. The results showed that combined application of 15 kinds of passivators increased the soil pH, soil cation exchange capacity (CEC) and soil organic matter by 0.25~1.04, 2.65%~50.96% and 0.22%~17.20%, respectively, and reduced the exchangeable Cr content by 35.21%~55.63%. Except for the combination of quicklime (L) + passivator 4 (D4) + chicken manure (M) which could significantly increase the soil reducible Cr, other combinations had no significant effect on soil reducible Cr. After application of passivator, soil oxidizable Cr and residual Cr had no significant change, content of exchangeable Cu decreased by 6.66~33.42% in the soil. soil reduciblu Cu and Oxidizable Cu had nosigificant change. The combination of 15 kinds of passivators increased the soil residual Cu content by 0.32%~5.04%, and the combinations of D3, L+D1+M and L+D4+M could significantly increase the content of soil residual fraction. Root system showed the highest enrichment capacity for Cr and Cu. After application of passivator, the contents of Cr in rice roots, straw, rice husk and brown rice decreased by 4.59%~49.41%, 39.84%~76.87%, 7.14%~36.10% and 17.32~67.10%, respectively. After application of passivator, the contents of Cu in rice roots, straw, rice husk and brown rice decreased by 10.57%~48.41%, 7.99%~52.53%, 21.12%~45.11% and 14.39%~66.92%, respectively. The combinations of 15 kinds of passivator could reduce the content of Cr and Cu in brown rice, and the combination of L+D2+M was the best.