Taking the "Hutai No.8" grape variety widely planted in Shaanxi Province as the test material, the first berry (fruiting at bearing shoot)and the secondary berry (fruiting at axillary shoot) quality indexes (such as soluble sugar, soluble protein, vitamin C, soluble solids, titratable acid, resveratrol and procyanidine) in different summer pruning intensities were measured in greenhouse. The results were used to synthetically evaluate the response of fruit quality to different summer pruning intensities, and to provide guidance for greenhouse grape cultivation and management. The results showed that summer pruning intensity could obviously impact the quality of the first berry and the secondary berry, and there were obvious differences between the quality indexes. In the case of fruiting secondary berry, the middle shoot pruning improved the quality of the first berry, but reduced the quality of the secondary berry. In the case of prohibiting secondary fruiting, the grape quality with the long shoot pruning was better. Although the taste and flavor of the first berry treated by the short shoot pruning was poor, its healthcare value was higher. The healthcare value of the secondary berry was generally better than the first berry, but its flavor was generally poor.