The pot buried experiments were conducted to study the effects of soil pH, nitrogen application, seasons and pakchoi planting on the degradation of bast fiber mulch film(BFMF) and the changes in soil microbial biomass and enzymes activities. The results showed that the degradation process of BFMF obeyed the Olson attenuation model (R²=0.689~0.996, P<0.05). The application of nitrogen and planting of pakchoi during the autum-winter season significantly inhibited the degradation of BFMF (P<0.05). Further, the degradation rate of BFMF was significantly higher during summer than autumn-winter season (P<0.05). While, limited effect of soil pH (5.80~8.34) on the degradation of BFMF was observed (P>0.05). Moreover, there were significantly positive relationships between the degradation rates of BFMF and the soil microbial biomass carbon, nitrogen (MBC, MBN), cellulose and catalase activities at the end of the experiments periods (P<0.05).These results indicated that the different degradation characters of BFMF might be due to the changes in soil microbial biomass and enzymes activities under different soil environment conditions.