A field experiment was conducted to study the yield of double cropping rice and its uptake of nitrogen (N), phosphorus (P) and potassium (K). The fertilizer utilization efficiencies and the relationships between the absorption and utilization efficiencies of P and K and N uptake and utilization were also measured under the different applications in N but the same in P and K. Our results showed that the rice yield was increased by moderate N application, however, high N showed negative effects on crop production. As for N application, 75%N treatment gained the highest yield for early rice, which was increased by 58.1% compared with CK without fertilizer application. And 100%N treatment had the highest yield for late rice, 67.6% more than CK. The accumulations of N, P and K in rice plants were significantly affected by N application levels. The accumulations of N, P and K in early and late rice grains were the highest under 100%N treatment, and their accumulations in rice plants were also higher than other treatments. Different nitrogen levels also had significant effects on N, P and K nutrient utilization efficiency. Our results showed a significant correlation between the accumulation of P and K and the accumulation of N in the early and late rice plants. The apparent use efficiencies and agronomy efficiencies of P and K were correlated positively with N accumulations in rice plants, respectively. Moderate N application could increase the yield of double cropping rice, promote the absorption of N, P and K by rice, and improve their utilization efficiencies. In this research, considering the yield effects of double cropping rice and the fertilizer utilization efficiencies, the suitable N application rate should be between 105 kg N/hm² and 146 kg N/hm².