A pot experiment was conducted to evaluate the effects of different biochars derived from coconut shell, bamboo sawdust, dead pig and tobacco stalk respectively (at 3% application rate) on the physicochemical properties of tobacco-planting soil, agronomic characteristics of tobacco plant and chemical composition of the flue-cured tobacco leaves. The results showed that the electrical conductivity, contents of available nutrients and organic carbon and enzyme activities of the tobacco-planting soil were influenced significantly by the application of different biochars. The highest content of soil organic carbon was observed in the bamboo biochar treatment, which increased by 146.37% compared to the control. Moreover, the application of pig biochar significantly (p<0.05) increased the electrical conductivity, available phosphorus and potassium, and promoted the activities of catalase and urease in tobacco-planting soil. The application of biochars had a slight influence on the agronomic traits of tobacco plant. The stem girth of tobacco plant in pig biochar treatment increased by 0.78 cm compared to the control. The biomass of tobacco plant significantly (p<0.05) increased, as the dry weight of tobacco leaves increased by 58.07% and 47.01% in the pig biochar and tobacco stalk biochar treatments, respectively, compared to the control. In addition, after the application of bamboo biochar, pig biochar and tobacco stalk biochar, the contents of nicotine, nitrogen, potassium and reducing sugar in flue-cured tobacco leaves all conformed to the standard of high quality tobacco. The application of pig biochar also significantly (p<0.05) increased the content of chloride in flue-cured tobacco leaves, and adjusted the sugar/nicotine and nitrogen/nicotine ratios to meet the standard of high quality tobacco. In conclusion, pig and tobacco stalk biochars were more effective than others in improving tobacco-planting soil properties and nutrient status, as well as promoting tobacco biomass and quality in the present study.