In order to explore the reasonable ratio of topdressing gluten wheat with nitrogen fertilizer, the effect of basal nitrogen application rate, dressing rate at jointing stage and booting stage (10:0:0, 7:3:0, 7:2:1, 6:4:0, 6:2:2, 5:5:0 and 5:3:2) on the N accumulate and transfer and grain yield and quality of CA0547 (strong gluten wheat) were studied in the irrigated wheat field in central of Shanxi Province. The results showed:(1) Appropriate dressing N had a significant regulatory effect on accumulation and translocation of N and dry matter, yield and quality of CA0547. (2) Dressing nitrogen could significantly increase nitrogen content after jointing stage, N transfer amount pre-anthesis and N accumulation amount post-anthesis, promote accumulation of N in grain, increase dry matter transport pre-anthesis and dry matter accumulation post-anthesis, which provided a material basis for the increase of yield. (3) About 68.38%~75.18% of N in the grains came from nitrogen translocation pre-anthesis, and 55.12%~70.04% of yield came from dry matter accumulation post-anthesis. Dressing nitrogen increased yield by increasing the number of ears and per spike, and increased N absorption and production efficiency. (4) Dressing nitrogen could improve the contents of gliadin, gluten, protein and wet-gluten, improve the gluten index and starch content, improve glutenin/gliadin ratio and straight/branch ratio, and improve grain quality. Correlation analysis also showed that increase dry matter accumulation post- anthesis and pre-anthesis nitrogen translocation amount of vegetative organs could improve quality. (5) The effect of N application at jointing and booting stage was inferior to N application at jointing stage. Comprehensive analysis showed that N amount of 150 kg/hm², at nitrogen ratio of 6:4:0 (base fertilizer:jointing fertilizer:boosting fertilizer) could better coordinate the relationship between yield and quality.