The effects of different kinds of organic manures (compost manure, chicken manure, cow manure, and pig manure) application on organic carbon (C) concentrations and humus composition in bulk soil and its different size aggregate fractions were studied based on field location experiment in a black soil with organic fertilization at the Jilin Academy of Agricultural Sciences. The results showed that, compared with no fertilizer (CK) and single application of chemical fertilizers (NPK), the application of organic manures in combination with chemical fertilizers increased significantly (P < 0.05) the concentrations of organic C, humic acid C (HAC) and humin C (HUC) in bulk soil. Meanwhile, the concentrations of organic and humic C within different size aggregate fractions also increased after the combined application of organic manures with chemical fertilizers compared to CK and NPK treatments, with significant increase for organic C, HAC and HUC within different size aggregate fractions following compost manure application. Among the experimental organic manures, the concentrations of organic C, HAC and HUC were higher for compost manure than for other organic manures treatments, with significant difference between compost manure and cow manure treatments. With respect to other organic manures treatments, > 0.25 mm aggregate-associated organic C concentration was higher after the application of compost manure and cow manure, with significant difference between compost manure and cow manure with chicken manure treatment for 2~0.25 mm aggregate fraction. Moreover, the concentrations of HAC and HUC in 2~0.25 mm aggregate fraction were significantly higher for compost manure than for pig manure treatment, whereas the concentrations of HAC 0.25~0.053, <0.053 mm aggregate fraction were significantly lower for compost manure than for chicken manure treatment. The above results indicated that the combined application of organic manure with chemical fertilizers increased the concentrations of organic and humus C within soil aggregates, but the effects of different organic manures were distinct.