The Fuji / M. hupehensis. Biennial plants were selected, and the different doses of vitamin B6 (VB6) and compound vitamin B (COB) were added under pot experiments to study the effects on biological characteristics and physicochemical properties of apple trees. The pot experiments were carried out in 2006. VB6 and COB were added into the soil of Fuji on 26th May. And the doses were 0 (control), 0.02, 0.03, 0.04 g/plant respectively.The plant height, diameter, leaf area, leaf antioxidant activity, chlorophyll content, photosynthetic fluorescence parameters and the growth and distribution of roots were determined. The results showed that different doses of VB6 and COB had different effects on plant height, stem diameter, plant biomass, leaf area and antioxidant activity, photosynthetic fluorescence parameters and root distribution of apple trees.The plant growth was significantly improved by the addition of VB6 and COB. COB of 0.04 g/plant treatment had the most significant effects on aboveground growth, and VB6 with 0.03 g/plant increased leaf area most significantly, i.e. 22%. The plant height, stem diameter and tip length were increased by 24%, 34%and 45% significantly, compared with the control in October, and no significant difference were observed between VB6 of 0.03 g/plant and COB of 0.04 g/plant. The total length, weight, volume and surface area of the underground were significant improved under VB6 of 0.03 g/plant and COB of 0.04 g/plant, and the number of root tip and bifurcation also increased significantly. There was no significant difference in antioxidant enzyme activities of apple leaves under VB6 of 0.02 g/plant and COB of 0.02 g/plant, compared with control. The resistance of plants was significantly enhanced under VB6 of 0.03 g/plant and COB of 0.04 g/plant, with the increased activities of antioxidant enzymes. The chlorophyll contents in apple leaves were significantly improved, and the photosynthetic fluorescence characteristics were also improved remarkably, and there was no significant difference between VB6 of 0.03 g/plant and COB of 0.04 g/plant. The results showed that VB6 of 0.03 g/plant and COB of 0.04 g/plant had the most significant effect on the biological traits and physicochemical properties of apple trees. In view of the higher effective dose and cost of COB, it was recommended to use VB6 to improve soil nutritional conditions.