In order to study the change of the SOC's,Nitrogen's,SMBC's and SMBN's contents in soil,a experiment on the long-term locating field plots was performed smoothly on the semi-humid areas of the west of the Henan province.The results suggested that organic carbon in soil and nitrogen in soil are both increased under the sub-soiling tillage and no-tillage.Comparing with the other tillage patterns,the sub-soiling tillage have a highest SOC contents in soil,that is 6.79 g/kg,13.82% improved comparing to the traditional tillage.Nitrogen contents in soil under no-tillage have a highest contents among the above tillage patterns that is 0.797 g/kg,10.42% improved comparing to the traditional tillage.SOC and nitrogen is decreasing with soil layer being deepened.In the 0~20 cm soil layer under the sub-soiling tillage and no-tillage,SMBC and SMBN contents have been 79.3%,19.9% and 17.92%,8.13% improved comparing to the traditional tillage,and then the phenomenon of the bacterial enrichment was occurred under the low sloping soil.The positive co-relation distinguishedly was appeared between the SMBC and nitrogen in soil,SMBN,SMBC,and SMBN can be considered as the biological indexes to estimate the quality of the soil,for which have a distinguished relation.Hence,the subsoiling tillage and no-tillage have perfect future in appliance.