A pot experiment was carried out to study the effect of slow-release fertilizers(SRF) on yield and quality of Trifolium pretense in strong acidic soil,acidic soil and neutral soil.As comparison to common compound fertilization(CCF),the results indicated that the yield of Trifolium pretense was increased significantly for the application of SRF in different soils,and in terms of the increase degree,the sequence of the soil was strong acidic soil(55.8%)> neutral soil(39.9%)> acidic soil(7.4%).The yield was differ from application of different SRF in different soil.The best yield was appeared for the application of SRF2 in strong acidic soil and acidic soil,while in neutral soil the highest yield was obtained for the application of SRF2.The forge nutritive quality and mineral quality was enhanced for the application of SRF in the first and second batch of Trifolium pretense.The crude protein(CP) was increased from 4.0%~23.1%,the ether extract(EE) was increased from 2.5%~38.6%,the crude ash(CA) was enhanced from 1.4%~23.6%,the acid detergent fiber(ADF) was decreased from 2.5%~23.3%,the element phosphorus was increased from 4.9%~18.6%,and calcium was increased from 1.8%~13.7%.The content of isoflavonoids in Trifolium pretense was strongly affected by different soils,in strong acidic soil and acidic soil the content of isoflavonoids was lower in treatments SRF than in treatment CCF,while in neutral soil the content of isoflavonoids in Trifolium pretense were all enhanced in different treatments.