The experiments were carried out on the farm of Henan agricultural university, during 2003-2004, using high-gluten cultivar Yumai 34 as material. This study was to clarify photosynthetic characteristics and grain yield variations with different N, P, K and S rates, so as to provide some experimental foundations for reasonable N, P, K and S fertilization of wheat. The results of the study revealed that under the field fertility of this experiment, rates of N, P, K and S had obvious adjust effect on canopy photosynthetic rate, chlorophyll content and nitrate reductase activity, and all photosynthetic characteristics indices increased with N, P, K and S supply, but the most powerful fertilizer factor at different date after anthesis was different. Combined fertilization of N, P, K and S also obviously regulated grain yield, and effect of S and N on yield was at 0.01 significant level, while effect of P and K was not. NTOP120K180S30 treatment was the optimum combination.