Abstract:This study was carried out to explore the reasonable utilization of brackish water in areas irrigated with brackish water and freshwater is short. This study set four treatments, including, reclaimed water irrigation(T1), mixed irrigation of 1∶2 with brackish water-reclaimed water (T2), mixed irrigation of 1∶1 with brackish water-reclaimed water (T3) and brackish water irrigation (T4), and took tap water irrigation (CK2), bare land irrigation with tap water (CK1) and bare land irrigation with 1∶1 with brackish water-reclaimed water (CK3) as the control, of which the saline degree of brackish water was 5 g/L. Based on the pot experiments, we measured the data of soil water content, soil salt content, water drop penetration time (WDPT), soil organic matter content, and soil enzyme activities including soil sucrase (S-SC), soil alkaline phosphatase (S-AKP/ALP), soil urease (S-UE), soil polyphenol oxidase (S-PPO), and analyzed the their responses to the mixed irrigation with different ratios of brackish water to reclaimed water. Finally, the index of integrated biological response version 2 was used to evaluate the impact of mixed irrigation on soil. The results indicated that: (1) Compared to T1, soil water content and soil salt content gradually increased significantly with the increase of the proportion of brackish water in the mixed solution on the whole. (2) Compared to T1, soil contents of K+ and Ca2+ had slight descending trend, soil contents of Na+ and Cl- increased obviously, soil SO42- content improved significantly, and soil Mg2+ content changed with no obvious trend with the increase of the proportion of brackish water in the mixed solution. (3) There were no significant differences in soil WDPTs and soil organ matter contents between T1 and CK2. Compared to T1, soil WDPTs and organic matter content had the ascending trend with the increase of the proportion of brackish water in the mixed solution. (4) The responses of different soil enzyme activities to different mixed irrigation with brackish water and reclaimed water were different. S-SC activity in T2 was the highest, S-UE activity in T3 was the highest but had no obvious differences from other treatments, and S-PPO activity in T4 was the highest following by T3 and no significant difference between them. (5) The index of IBRv2 in T2 was the lowest, and the value was 6.89. Therefore, considering soil environmental indexes and the limits of reclaimed water, reclaimed water could be used as an alternative source of fresh water in combination with brackish water, and mixed irrigation of 1∶2 with brackish water-reclaimed water is recommended based on the index of IBRv2 in short fresh water resource and areas irrigated with brackish water.