Abstract:It is an effective way to alleviate the scarcity of fresh water resources to use unconventional water resources. In areas rich in brackish water, the freshwater resources are generally inadequate, but there are abundant reclaimed water resources. In order to explore the reasonable utilization of brackish water and reclaimed water in areas where freshwater was scarce, by using Shanghai green pot experiment, this research studied the soil salt-water dynamics, crop biomass including aboveground biomass and underground biomass, and crop physiological characteristics such as chlorophyll content of leaves, soluble protein content, malondialdehyde (MDA) content, activities of catalase(CAT), superoxide dismutase (SOD), and peroxidase (POD) under mixed irrigation of brackish water and reclaimed water at different ratios. There were four treatments, including reclaimed water irrigation (T1), mixed irrigation of brackish water and reclaimed water at 1∶2 (T2), mixed irrigation of brackish water and reclaimed water at 1∶1 (T3) and brackish water irrigation (T4), taking the fresh water irrigation (CK) as control. The saline degree of brackish water was 3 g/L. The results indicated that: (1) Both soil water content and soil salt content were improved under different mixed irrigation compared with CK, among which, the difference of soil water content under T4 and CK reached a significant level (P<0.05), and no difference between T1, T2, T3 and CK. While, soil salt contents in all mixed irrigation treatments had significant differences with CK. Compared with T1, soil water content and soil salt content (except T2 and T3) gradually increased significantly with the increase of brackish water proportion in the mixed solution. (2) Mixed irrigation with brackish water and reclaimed water affected aboveground fresh weight of crop while mixed irrigation had no obvious effects on aboveground dry weight and underground biomass. Compared with T1, aboveground fresh weights under T2, T3, and T4 decreased by 24.78% to 26.36% significantly, aboveground dry weights reduced by 19.14% to 24.54% insignificantly, and the fresh and dry weight of underground changed slightly. (3) Mixed irrigation with brackish water and reclaimed water promoted the activity of SOD significantly, but had no obvious effects on crop physiological characteristics including chlorophyll content, soluble protein content, MDA content, POD activity and CAT activity. Compared with T1, chlorophyll-a content decreased by 4.98%, 3.82% and 9.26%, chlorophyll-b content reduced by 10.88%, 8,20% and 13.46%, total chlorophyll content decreased by 9.76%, 6.12% and 10.15%, CAT activity improved by 8.51%, 8.51% and -19.15%, POD activity increased by 1.92%, 17.24% and -2.87%, and SOD activity improved by 104.07%, 62.20% and 41.67% respectively under T2, T3 and T4. The soluble protein content decreased first and then increased while MDA content increased first and then decreased with the increase of brackish water proportion in mixed solution. Therefore, considering soil water-salt dynamic, crop physiological characteristics and the limits of reclaimed water, it could be used as an alternative source of fresh water to mix with brackish water (3 g/L), the suitable mixed proportion would be 1∶1 based on the integrated biological response version 2 (IBRv2). The research results could provide the reference for the utilization of brackish water in the areas where fresh water is short.