In order to explore the effects of different irrigation methods on soil structure and nutrients in kiwifruit orchard, two treatments of surface irrigation and micro-sprinkler irrigation were applied in the kiwifruit orchard of Yangling, Shaanxi Province from 2017 to 2019. The structure of soil aggregates and the contents of organic matter, available nitrogen, available phosphorus and available potassium in 0-50 cm soil layer were determined. The results showed that in 0-50 cm soil layer, the distribution uniformities of the soil particles size of >5.0, 2.0~5.0, 1.0~2.0, 0.5~1.0, 0.25~0.5, <0.25 mm in different soil layers under surface irrigation were higher than those under micro-sprinkler irrigation, and the grain of <0.25 mm was the most uniform in the two irrigation methods. The content of water-stable aggregates (> 0.25 mm) in soil under micro-irrigation was significantly higher than that under surface irrigation. The mean weight diameter (MWD) and geometric mean diameter (GMD) of soil aggregates under micro-sprinkler irrigation were higher than those under surface irrigation, while the fractal dimension (D) was lower than that under surface irrigation. The total contents of N, P and K in soil under micro-sprinkler were higher than those under surface irrigation. Micro-sprinkler irrigation could maintain the stability of soil structure and soil fertility, and promote the sustainable development of soil. It is a suitable irrigation method for mature kiwifruit orchard.