Research on soil hydraulic properties in different positions of the hillslope can provide reference for the prediction of surface hydrological process and ecological restoration in mountainous areas. In this study, undisturbed soil samples were collected from different slope positions in Dalaoling forest region, which was a typical small catchment of the Three Gorges Reservoir area, and the hydraulic parameters such as saturated water conductivity, equivalent pore diameter and water storage capacity were measured. The V-G model was used to fit the soil water characteristic curve to evaluate the soil permeability and water holding capacity of soil at different slope positions. The results showed that the soil infiltration performance of the top position was the best, and the average saturated hydraulic conductivity was 108.54 cm/d, while the average saturated hydraulic conductivity of other four slope positions was 34.81~47.56 cm/d. However, the water content of the top position decreased largely (63.67%) when the suction value increased from 0 to 100 kPa, while the average water content of other four positions decreased by 46.36%~52.07%. The macropore volume ratio of the soil at the top position was 20.95%, and that of the other four points was ranging from 12.27%~16.58%. The percentage of field water capacity in total soil water storage capacity was 45.24% in the top position, which was the smallest, and that of the other four points was 60.08%~65.33%. The V-G model was used to fit the soil water retention curve, and the determination coefficient (R²) of each point was greater than 0.95, which indicated that the fitting effect was good. The saturated hydraulic conductivity was positively correlated with soil macropore volume ratio and sand content, and negatively correlated with silt content and clay content. The parameter α of V-G model was related to air entry values. The smaller α value was, the stronger the water retention capacity was. In the experiment, α value was positively correlated with sand content and macropore volume ratio, and negatively correlated with silt content and clay content. The more sand particles and macropores was, the worse soil water holding capacity was. The value of parameter n affected the curvature of fitting water characteristic curve. The smaller n value was, the smoother the curve was. And n value was positively correlated with sand content and negatively correlated with silt content. The more sand content was, the faster the soil drainage was with the increasing of suction. The soil at the top of the mountain slope had the worst water-holding capacity and the strongest permeability, while the difference of other slope positions was not significant. The results of V-G model fitting were consistent with the measured results.