To reveal the water-uptake patterns of different plant species in subtropical humid area and to understand the hydrological cycle of regional forest system, the typical evergreen plant species Cinnamomum camphora, Cunninghamia lanceolate, and Camellia pitardii in Changsha were used as research objects by using stable isotope techniques and Iso-source Mixed Model for quantitative analysis of the water-uptake patterns of different plant species and their responses to precipitation. The results showed that the variations of δD in water from 0-10 cm soil layer and the δD in precipitation are basically identical, and the two kind of δD become more positive with time gradually during the observation period from September 2018 to February 2019. With the increase in depth, the influence of precipitation on δD in soil water gradually reduced and tended to be stable. During September to November, the reflection to precipitation of the three plant species became smaller. The δD in the plant stem water did not show more positive in line with the δD in precipitation. From December to the next February, the three plant species responded sensitively to precipitation. The variation of δD in plant stem water was consistent with the change of δD in precipitation. During the observation period, the water use depth of these plants changed from deep to shallow with time. In September, these plants mostly used 60-100 cm soil water, and the proportion exceeded 65%. During October to November, there were obvious differences in the water-uptake patterns of these plants. The water use of C. camphora from the four soil layers for 0-10, 10-20, 20-60 and 60-100 cm was relatively uniform, and the proportion was 28.2%, 23.5%, 22.0%, and 26.3% respectively; C. lanceolate primarily used 0-10 and 60-100 cm soil water, and the proportion was 56.4% and 22.2% respectively; C. pitardii principally absorbed 0-20 cm shallow soil water, the proportion was 67.4%. From December to February of the next year, these plants generally used 0-10 cm soil water, and the proportion was above 70%.