Abstract:A pot experiment was carried out to study the effects of applying phytol to lead(Pb)-contaminated soil on soil enzyme activity and Pb availability, and the alleviation effects of phytol to the Pb-contaminated soil under different treatment time were discussed. The results showed that adding phytol to soil in a short time promoted the activities of soil catalase, sucrase and alkaline phosphatase, while inhibited soil urease activity. Moreover, each enzyme had different sensitivity to phytol, among which alkaline phosphatase was the most sensitive. With the prolongation of treatment time, the activities of catalase and urease as well as available Pb content in the soil decreased, while the activities of invertase and phosphatase increased significantly. When the Pb concentration was 600 mg/kg in soil and the phytol concentration was 50 mg/kg, sucrase activity was the strongest, which was 122.28% higher than that of the blank control. Additionally, when the phytol concentration was 250 mg/kg in the soil, the activity of phosphatase was the strongest, which was 251.61% of the blank control. In conclusion, the addition of phytol to Pb-contaminated soil could change soil enzyme activity and reduce the content of available Pb in the soil. What's more, with the prolongation of culture time, the alleviation effect of phytol on Pb-contaminated soil reduced gradually.