Anaerobic soil disinfestation (ASD) as an alternative chemical fumigation of soil, has been gradually popularized in various places. However, effects of ASD is dependent on types of soil and amendments added. A field experiment was conducted to study the effects of ASD with different amendments on soil properties and microbial community, which would provide a scientific basis for environmentally friendly soil disinfestation method in solar greenhouse. The results showed that (1) the treatments except for ammonium bicarbonate treatment (AB) significantly decreased soil EC in 0-20 cm soil layer, but only irrigation without amendments (CK treatment) significantly increased soil EC, NO₃^--N in 20-40 cm soil layer. AB treatment significantly increased NH₄⁺-N in 0-20 cm soil layer and NO₃^--N in 20-40 cm soil layer. And the addition of chicken manure (CM treatment) significantly increased the NH₄⁺-N, pH, organic matter, total nitrogen and available nutrients, and significantly decreased the accumulation of NO₃^--N and EC in 0-20 cm soil layer. (2) CM treatment significantly decreased soil bacterial richness and evenness. However, the relative abundance of plant growth-promoting bacteria (PGPR), Bacillus and Pseudomonas increased significantly. For the fungal community, the diversity and abundance of fungi in different treatments were not significantly different from those of before treating. However, the relative abundance of pathogen-Fusarium in AB, PS and CM treatment decreased significantly. The relative abundance of deneficial Aspergillus of CM treatment significantly increased. (3) Considering soil physical, chemical and microbial properties, chicken manure as an organic carbon source, had more positively effects of disinfestation, not only increased its decomposition, but also easy to get. Meanwhile, the application of chicken manure would be helpful for implementing double reduction of fertilizer and pesticides.