Abstract:Biological simulation of the hydroponic experiment was conducted to explore the effects of spraying eight different foliar silicon solutions on total arsenic (As) accumulation and As speciation in rice seedling. The results showed that the application of foliar silicon conditioner reduced As content in rice roots and shoots. Moreover, As contents in roots and shoots of rice seedlings treated with Na2SiO3+rhamnolipid (Si6) were the lowest, 10.04 mg/kg and 0.31 mg/kg, respectively, which were 25.35% and 33.10% lower than those of the control (P<0.05), respectively. As (Ⅲ) was the predominant speciation of As in rice. Compared to the control, foliar application of solution with Na2SiO3+rhamnolipid significantly reduced the As (Ⅲ) contents in roots and shoots by 27.74% and 21.50%(P<0.05), respectively. Considering the accumulation of As in rice roots and shoots and the transport of As from roots to shoots, the cluster analysis of different silicon conditioner treatments showed that the treatment of spraying Na2SiO3+rhamnolipid was a low accumulation group of As in rice. Therefore, the rhamnolipid as bio-surfactant combined with silicate solution was the best silicon conditioner for inhibiting the uptake and accumulation of As in rice seedlings, which would provide technical support for food security of rice in As-contaminated regions of China.