文章摘要
张婷婷, 朱叶心怡, 吴玉环, 张琪, 叶润, 崔乐怡, 陈瑞, 陶涛, 刘鹏.镉胁迫下外源IAA对栝楼生理变化和耐镉性的影响[J].水土保持学报,2019,33(4):335~341
镉胁迫下外源IAA对栝楼生理变化和耐镉性的影响
Effects of Exogenous IAA on Physiological Changes and Cadmium Tolerance of Trichosanthes kirilowii Maxim. Under Cadmium Stress
投稿时间:2018-12-29  
DOI:10.13870/j.cnki.stbcxb.2019.04.047
中文关键词: IAA  镉胁迫  栝楼  生长特性  光合特性  抗氧化酶
英文关键词: IAA  cadmium stress  Trichosanthes kirilowii Maxim.  growth characteristics  photosynthetic characteristics  antioxidant enzyme
基金项目:国家自然科学基金项目(41571049,30540056);浙江省公益技术农业项目(2011C22053)
作者单位E-mail
张婷婷1, 朱叶心怡1, 吴玉环2,3, 张琪1, 叶润1, 崔乐怡1, 陈瑞1, 陶涛1, 刘鹏1 1. 浙江师范大学植物学实验室, 浙江 金华 321004

2. 杭州师范大学生命与环境科学学院
, 杭州 310036

3. 中国科学院沈阳应用生态研究所
, 沈阳 110016 
sky79@zjnu.cn 
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中文摘要:
      为探究IAA对镉胁迫下栝楼(Trichosanthes kirilowii Maxim.)生理响应及耐镉性的影响,以保定栝楼(耐镉品种)和浦江栝楼(镉敏感品种)为材料,进行土培试验,研究800 μmol/L镉胁迫下不同浓度IAA (10,35,60 μmol/L)对2个栝楼品种的生长特性、光合特性、抗氧化酶活性及丙二醛(MDA)含量的影响。结果表明:(1)与CK组相比,800 μmol/L Cd处理下,保定和浦江栝楼的平均根长、地下部分鲜重、超氧化物歧化酶(SOD)活性、叶绿素含量、最大光化学效率(Fv/Fm)和电子传递速率(ETR)明显降低,初始荧光(Fo)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及MDA含量显著增高,细胞膜脂过氧化程度加深;(2)随IAA浓度升高,保定栝楼平均根长与地下部分鲜重持续增长,最大增幅为24.83%和40.55%,而浦江栝楼则由上升转为下降趋势;(3)外源IAA加入后,2个栝楼品种幼叶叶绿素a、叶绿素b、叶绿素a+b含量、叶绿素a/b、叶绿素荧光参数中的Fv/Fm、ETR及抗氧化酶SOD、POD、CAT活性均呈现出先升后降的趋势,Fo和MDA含量先降后升,其中,35 μmol/L的IAA使叶绿素a、叶绿素b、叶绿素a+b含量、叶绿素a/b、Fv/Fm、ETR及SOD、CAT活性上升到峰值,而MDA含量降低35.44%~43.23%,达到谷值;(4)由隶属函数均值耐镉性分析得知,不同浓度IAA对栝楼镉毒害的缓解程度为35 μmol/L > 10 μmol/L > 60 μmol/L > 0 μmol/L。由此可见,外源IAA的施加对于缓解植物镉胁迫具有一定的应用前景。
英文摘要:
      In order to investigate the effects of IAA on physiological response and cadmium tolerance of Trichosanthes kirilowii Maxim. under cadmium stress, Baoding T. kirilowii (cadmium-tolerant) and Pujiang T. kirilowii were selected as experimental materials, and soil culture experiments were conducted to determine the effects of exogenous IAA (10, 35 and 60 μmol/L) on physiological changes, photosynthetic characteristics, antioxidant enzyme activity and malondialdehyde (MDA) content of these two varieties under 800 μmol/L cadmium stress. The results showed that:(1) Compared with the CK, the average root length, fresh weight of underground part, superoxide dismutase (SOD) activity, chlorophyll content, maximum photochemical efficiency (Fv/Fm) and electron transfer rate (ETR) of the two kinds of T. kirilowii significantly decreased under 800 μmol/L Cd treatment, while the initial fluorescence (Fo), peroxidase (POD) activity, catalase (CAT) activity and MDA content increased significantly, and the lipid peroxidation of cell membrane deepened. (2) With the increasing of IAA concentration, the average root length and fresh weight of underground part of Baoding T. kirilowii increased continuously, with the maximum increase of 24.83% and 40.55%, while those of Pujiang T. kirilowii changed from ascending to descending. (3) After the addition of IAA, the content of chlorophyll a, b, a+b and chlorophyll a/b, Fv/Fm, ETR, SOD, POD and CAT activities in the young leaves of the two varieties increased first and then decreased, while Fo and MDA content dropped firstly and then increased. The content of chlorophyll a, b, a+b, a/b, Fv/Fm, ETR, SOD and CAT increased to the peak values with the addition of IAA at 35 μmol/L, while MDA decreased by 35.44%~43.23%, reaching the valley value. (4) The analysis of membership function indicated that the ability of cadmium tolerance of different IAA concentrations was 35 μmol/L > 10 μmol/L > 60 μmol/L > 0 μmol/L. Therefore, applying exogenous IAA has a certain application prospect in alleviating cadmium stress in plants.
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