In order to reveal the effects of different reclamation years and fertilization measures on nitrogen transformation characteristics of reclaimed soils in coal mining subsidence areas, intermittent leaching and aerobic culture method, laboratory aerobic incubation method with constant temperature and controlled humidity, and nitrate loss method were used to study the regularities of soil mineralization, nitrification and denitrification in reclaimed soil for four years and eight years, respectively, under five fertilization measures. The results showed that as the increasing of reclamation years, both cumulative mineralized nitrogen (N_t) and potentially mineralizable nitrogen (N₀) increased, while mineralization rate (N_t/N) and the proportion of N₀ to total N (N₀/N) did not change significantly. Under different fertilization measures, the N_t, N₀, N_t/N and N₀/N of eight years reclaimed soil in MCFB treatment (application chemical fertilizer combined with biological organic fertilizer) increased by 65.22%,65.21%, 60.42% and 60.76%, respectively, compared with chemical fertilizer treatment (CF). The soil nitrification rate and the time needed to reach the maximum nitrification rate (T_max) were less affected by reclamation years, and nitrification rates of different fertilization treatments were all higher than that of CK treatment, but there was no significant difference between treatments. The maximum nitrification rate (V_max) increased with the increasing of reclamation years. Treatments of MCFB could improve V_max and T_max more effectively than other treatments in soils reclaimed for four and eight years. Nitrate loss and nitrate disappearance rate increased with the increasing of reclaimation years. After seven days of cultivation, in the reclaimed soil for four years, the nitrate loss was the highest in organic manure treatment(M), reaching 78.72%, and the nitrate disappearance rate was the lowest in MCFB treatment, which was consistent with CK. There was no significant difference in soil nitrogen denitrification among different treatments for soil reclaimed for eight years. The sequence of short-term nitrogen transformation intensity was nitrification>denitrification>mineralization. On the whole, fertilization had obvious improvement effect on nitrogen transformation characteristics in reclaimed soil, and chemical fertilizer combined with biological organic fertilizer was more conducive to the maintenance and improvement of soil available nitrogen and reduce nitrogen loss.