The activity of soil urease, invertase, alkaline phosphatase, dehydrogenase and catalase were studied during the process of natural vegetation succession on the Yunwu mountain area. The result showed that. The activity of soil invertase, urease, alkaline phosphatase and dehydrogenase are all increase along with the vegetation rehabilitation ages. But soil catalase has no obvious increasing with it. In the plant community which was enclosed 23 years, soil sucrase, urease, neutral phosphatase, dehydrogenase enhanced significant, especially soil sub-layer. In the plant community which was enclosed 78 years, Soil sucrase, urease are highest which showed that the turnover of soil C and N are very active. Soil urease and invertase, alkaline phosphatase, dehydrogenase are positive relative, and the activity of these enzymes can reflect the vegetation rehabilitation in the certain extent, also natural revegetation play an important role in enhancing soil biological quality.